Species Publications

Publication details

Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining. (2016) PLoS Pathog 12, e1005685.
Wang G, Liu Z, Lin R, Li E, Mao Z, Ling J, Yang Y, Yin WB, Xie B
Abstract: Purpureocillium lilacinum of Ophiocordycipitaceae is one of the most promising and commercialized agents for controlling plant parasitic nematodes, as well as other insects and plant pathogens. However, how the fungus functions at the molecular level remains unknown. Here, we sequenced two isolates (PLBJ-1 and PLFJ-1) of P. lilacinum from different places Beijing and Fujian. Genomic analysis showed high synteny of the two isolates, and the phylogenetic analysis indicated they were most related to the insect pathogen Tolypocladium inflatum. A comparison with other species revealed that this fungus was enriched in carbohydrate-active enzymes (CAZymes), proteases and pathogenesis related genes. Whole genome search revealed a rich repertoire of secondary metabolites (SMs) encoding genes. The non-ribosomal peptide synthetase LcsA, which is comprised of ten C-A-PCP modules, was identified as the core biosynthetic gene of lipopeptide leucinostatins, which was specific to P. lilacinum and T. ophioglossoides, as confirmed by phylogenetic analysis. Furthermore, gene expression level was analyzed when PLBJ-1 was grown in leucinostatin-inducing and non-inducing medium, and 20 genes involved in the biosynthesis of leucionostatins were identified. Disruption mutants allowed us to propose a putative biosynthetic pathway of leucinostatin A. Moreover, overexpression of the transcription factor lcsF increased the production (1.5-fold) of leucinostatins A and B compared to wild type. Bioassays explored a new bioactivity of leucinostatins and P. lilacinum: inhibiting the growth of Phytophthora infestans and P. capsici. These results contribute to our understanding of the biosynthetic mechanism of leucinostatins and may allow us to utilize P. lilacinum better as bio-control agent.

Pochonia chlamydosporia 170

[ Opisthokonta | Fungi | Dikarya | . . .
. . . | Clavicipitaceae | Pochonia | Pochonia chlamydosporia ]

Pochonia_chlamydosporia_170 Picture Source
Taxonomy:Eukaryota | Opisthokonta | Fungi | Dikarya | Ascomycota | saccharomyceta | Pezizomycotina | leotiomyceta | sordariomyceta | Sordariomycetes | Hypocreomycetidae | Hypocreales | Clavicipitaceae | Pochonia | Pochonia chlamydosporia
Synonym:Metacordyceps chlamydosporia, Verticillium chlamydosporium var. chlamydosporium, Verticillium chlamydosporium, Pochonia chlamydosporia var. chlamydosporia, Diheterospora chlamydosporia var. chlamydosporia, Diheterospora chlamydosporia, Cordyceps chlamydosporia
Ncbi Go to NCBI Taxonomy (1380566)
Eol Encyclopedia of life
Wiki Wikipedia

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Purpureocillium lilacinum PLBJ-1

[ Opisthokonta | Fungi | Dikarya | . . .
. . . | Hypocreales | Ophiocordycipitaceae | Purpureocillium ]

Purpureocillium_lilacinum_PLBJ_1 Picture Source
Taxonomy:Eukaryota | Opisthokonta | Fungi | Dikarya | Ascomycota | saccharomyceta | Pezizomycotina | leotiomyceta | sordariomyceta | Sordariomycetes | Hypocreomycetidae | Hypocreales | Ophiocordycipitaceae | Purpureocillium
Synonym:Purpureocillium lilacinus, Penicillium lilacinum, Paecilomyces lilacinus
Ncbi Go to NCBI Taxonomy (33203)
Eol Encyclopedia of life
Wiki Wikipedia

Genome files Show genomes


Purpureocillium lilacinum PLFJ-1

[ Opisthokonta | Fungi | Dikarya | . . .
. . . | Hypocreales | Ophiocordycipitaceae | Purpureocillium ]

Purpureocillium_lilacinum_PLFJ_1 Picture Source
Taxonomy:Eukaryota | Opisthokonta | Fungi | Dikarya | Ascomycota | saccharomyceta | Pezizomycotina | leotiomyceta | sordariomyceta | Sordariomycetes | Hypocreomycetidae | Hypocreales | Ophiocordycipitaceae | Purpureocillium
Synonym:Purpureocillium lilacinus, Penicillium lilacinum, Paecilomyces lilacinus
Ncbi Go to NCBI Taxonomy (33203)
Eol Encyclopedia of life
Wiki Wikipedia

Genome files Show genomes


diArk 3.0 Content Info

Species:5699
References:167
Projects:8087
gen DNA Projects:4486
EST/cDNA Projects:849
Publications:2177
link to cymobase
link to scipio
link to www.motorprotein.de
MPI for biophysical chemistry

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